Glycophorin A (GYPA) (N-term) Mouse Monoclonal Antibody [Clone ID: HIR2]

CAT#: SM3141P

Glycophorin A (GYPA) (N-term) mouse monoclonal antibody, clone HIR2, Purified

Product Images

Surface staining of human peripheral blood with anti-CD235a (HIR2) purified, GAM-APC.

Specifications

Product Data

• Clone Name: HIR2
• Applications: AGG, FC, IHC
• Recommended Dilution: Flow Cytometry: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 µg per million cells.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Sections.
  Agglutination: Clone HIR2 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (Human, N-terminal)
• Specificity: This antibody Clone HIR2 recognizes N-terminal portion of Glycophorin A (and weakly of glycophorin B).
  Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: glycophorin A (MNS blood group)
• Database Link:
  Entrez Gene 2993 Human
• Background: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
• Synonyms: Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein