SOCS3 Mouse Monoclonal Antibody [Clone ID: SO1]

CAT#: SM3145P

SOCS3 mouse monoclonal antibody, clone SO1, Purified

Product Images

Western Blotting analysis (reducing conditions) of whole cell lysate of HeLa human cervix carcinoma cell line. Lane 1: immunostaining with Isotype mouse IgG2b control Lane 2: immunostaining with anti-SOCS3 (SO1)

Specifications

Product Data

• Clone Name: SO1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (Reducing conditions):
  Positive Control: Use HeLa human cervix carcinoma cell line.
  Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including protease inhibitor cocktail). Incubate 60 min on ice, centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
  This Clone SO1 is reported to work on Paraffin Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length SOCS3 protein.
• Specificity: This antibody SO1 reacts with SOCS3, an intracellular Cytokine Signaling Inhibitor.
• Formulation: PBS with 15 mM Sodium Azide as preservative, approx. pH 7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein A
• Database Link:
  Entrez Gene 9021 Human
• Background: SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.
• Synonyms: SOCS-3, CIS-3, SSI3, SSI-3