CD14 Mouse Monoclonal Antibody [Clone ID: MEM-15]

CAT#: SM3163B

CD14 mouse monoclonal antibody, clone MEM-15, Biotin

Product Images

Surface staining of human peripheral blood cells with anti-human CD14 (MEM-15) biotin / streptavidin-APC

Specifications

Product Data

• Clone Name: MEM-15
• Applications: FC
• Recommended Dilution: Flow Cytometry (1/1000).
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
• Specificity: The antibody MEM-15 reacts with human CD14 antigen, a 53 kDa glycoprotein expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
• Formulation: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4
  Label: Biotin
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
• Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a
  reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between
  lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
• Synonyms: CD14