CD8A Rat Monoclonal Antibody [Clone ID: YCATE55.9]

CAT#: SM411F

CD8A rat monoclonal antibody, clone YCATE55.9, FITC

Product Images

Staining of canine peripheral blood lymphocytes with RAT ANTI CANINE CD8:FITC.

Specifications

Product Data

• Clone Name: YCATE55.9
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Canine
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Canine CD8a chimaeric human IgG1 Fc fusion protein. Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line.
• Specificity: This antibody, clone YCATE 55.9 was clustered as CD8 in the first canine leukocyte antigen workshop. The antibody reacts with canine CD8a transfectants. Clone YCATE 55.9 is reported to block MHC class I dependant T-cell responses in vitro and in vivo.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Database Link:
  Entrez Gene 403157 Dog
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL