CD45 Rat Monoclonal Antibody [Clone ID: YKIX716.13]

CAT#: SM415APC

CD45 rat monoclonal antibody, clone YKIX716.13, APC

Product Images

SM415APC staining of canine peripheral blood lymphocytes.

Specifications

Product Data

• Clone Name: YKIX716.13
• Applications: FC
• Recommended Dilution: Flow Cytometry (Neat).
• Reactivities: Canine
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Canine thymocytes.
  Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
• Specificity: Clustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop: immunoprecipitates antigen of 180/200kD from Con-A blasts (1).
  This antibody reacts with CD45 on all outbred mongrels and beagles tested.
  Rat anti Dog CD45 antibody, clone YKIX716.13 recognizes Canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW).
  Clone YKIX 716.13: immunoprecipitates an antigen of 180/200kD from Con-A blasts (Cobbold et al. 1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform.
• Formulation: PBS
  Label: APC
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: Allophycocyanin
• Reconstitution Method: Restore with 1 ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: APC
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: CD45, PTPRC, L-CA, LCA, EC 3.1.3.48, T200, Leukocyte common antigen