MHC Class II Rat Monoclonal Antibody [Clone ID: YKIX334.2]

CAT#: SM421F

MHC Class II rat monoclonal antibody, clone YKIX334.2, FITC

Product Images

Staining of Canine peripheral blood lymphocytes with Rat anti-Canine MHC CLASS II Antibody -FITC Cat.-No SM421F (Clone YKIX334.2)

Specifications

Product Data

• Clone Name: YKIX334.2
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl Neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Canine
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Con A activated canine peripheral blood cells. Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
• Specificity: This antigen recognises MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Synonyms: MHC Class 2, HLA Class II