MHC Class II Rat Monoclonal Antibody [Clone ID: YKIX334.2]

CAT#: SM421PS

MHC Class II rat monoclonal antibody, clone YKIX334.2, Purified

Specifications

Product Data

• Clone Name: YKIX334.2
• Applications: FC, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  Flow Cytometry: Use 10 µl 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Canine
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Con A activated canine peripheral blood cells. Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
• Specificity:
  This antibody recognises monomorphic Canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop.
  The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In Dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Synonyms: MHC Class 2, HLA Class II