CD45 / LCA Mouse Monoclonal Antibody [Clone ID: CC1]

CAT#: SM442F

CD45 / LCA mouse monoclonal antibody, clone CC1, FITC

Product Images

SM442F (MOUSE ANTI BOVINE CD45:FITC) staining of bovine peripheral blood lymphocytes.

Specifications

Product Data

• Clone Name: CC1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use Neat-1/10 of diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Bovine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
• Specificity: This antibody reactive on the Bovine homologue of the Human CD45 antigen and is expressed on pan leucocyte cells.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200