IL6 Mouse Monoclonal Antibody [Clone ID: 4B6]

CAT#: SM481

IL6 mouse monoclonal antibody, clone 4B6, Purified

Specifications

Product Data

• Clone Name: 4B6
• Applications: ELISA, FC, WB
• Recommended Dilution: Western blotting:
  Flow cytometry: (Requires membrane permeabilization).
  ELISA: 5 µg/ml as a coating antibody.
  Use in combination with SP1250 in a Sandwich ELISA assays for Ovine IL-6.
• Reactivities: Monkey, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Ovine IL-6
• Specificity: Recognises ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells.
  Clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.
• Formulation: PBS pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: 1.0 mg/ml
• Background: Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.
  
  IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.
• Synonyms: IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF