CD45 / LCA Mouse Monoclonal Antibody [Clone ID: K252-1E4]

CAT#: SM490FC

CD45 / LCA mouse monoclonal antibody, clone K252-1E4, FITC

Product Images

Staining of pig peripheral blood with Mouse anti Pig CD45:FITC (SM490FC)

Specifications

Product Data

• Clone Name: K252-1E4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine Peripheral Blood lymphocytes.
  Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line.
• Specificity: This antibody recognizes all Porcine leucocytes.
  This K252.1E4 antibody recognizes an epitope common to all porcine CD45 isoforms (Schnitzlein et al. 1998).
  K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermann et al. 1994).
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity Chromatography on Protein A
• Conjugation: FITC
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200