Tubulin (TUBA1B) Rat Monoclonal Antibody [Clone ID: YOL1/34]

CAT#: SM568P

Tubulin (TUBA1B) rat monoclonal antibody, clone YOL1/34, Purified


USD 580.00

5 Days*

Size
    • 500 ug

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Specifications

Product Data
Clone Name YOL1/34
Applications ELISA, IF, IHC, IP, R, WB
Recommended Dilution ELISA: 10 µg/ml as Detecting antibody.
Radioimmunoassay.
Western blotting.
Immunofluorescence.
Flow Cytometry (Not tested).
Immunohistochemistry on Frozen and Paraffin Sections.
Reactivities Birds, Drosophila, Human, Mammalian, Mouse, Rat, Yeast
Host Rat
Isotype IgG2a
Clonality Monoclonal
Immunogen Yeast Tubulin.
Spleen cells from immunised LOU rats were fused with cells of the rat YB2/0 Myeloma cell line.
Specificity SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P).
This antibody is routinely tested in ELISA on Tubulin.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction.
Preservative: 0.09% Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography on Protein G
Gene Name Homo sapiens tubulin alpha 1b (TUBA1B)
Background Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.
Synonyms Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1
Reference Data
Protein Families Druggable Genome
Protein Pathways Gap junction, Pathogenic Escherichia coli infection

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