CD44 Mouse Monoclonal Antibody [Clone ID: W4-86]

CAT#: SM607PS

CD44 mouse monoclonal antibody, clone W4-86, Purified

Product Images

Rabbit peripheral blood granulocytes stained with CD44 Antibody (Clone W4/86) followed by Goat anti Mouse IgG Antibody -FITC.

Specifications

Product Data

• Clone Name: W4-86
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/25-1/200 diluted antibody to label 10⁶ cells in 100 µl.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: RL-5 T cell line glycoproteins. Spleen cells from an immunised mouse were fused with cells of the mouse P3.X63 Ag8 myeloma cell line.
• Specificity: This antibody recognises the CD44 cell surface antigen, a 90kD glycoprotein expressed by all leucocytes.
  In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly. Reactivity in Flow cytometry may be weak.
  Clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
• Synonyms: LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1