GAD67 (GAD1) (+ GAD2 / GAD65) Rabbit Polyclonal Antibody

CAT#: SP1218P

GAD67 (GAD1) (+ GAD2 / GAD65) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/1000-1/10000.
  Immunohistochemistry on Frozen Sections: 1/200-1/5000.
  Recommended Positive Control: Brain.
• Reactivities: Feline, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide sequence from the C-terminus of GAD
• Specificity: This antibody recognises both molecular forms of Glutamic Acid Decarboxylase (GAD65 and GAD67), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  State: Purified
  State: Liquid purified Immunoglobulin-rich fraction.
• Purification: Ammonium Sulphate Precipitation.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glutamate decarboxylase 1 (GAD1), transcript variant GAD67
• Database Link:
  Entrez Gene 14415 MouseEntrez Gene 24379 RatEntrez Gene 2571 Human
• Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.
• Synonyms: Glutamate decarboxylase 1, GAD-67
• Note: Predicted Molecular Weight: 65 and 67 kDa.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus