Calcr Rabbit Polyclonal Antibody

CAT#: SP1338PS

Calcr rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: ELISA: 1/10,000.
  Wewstern Blot (Reducing conditions).
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: 1/10-1/50.
  Immunohistochemistry on Paraffin Embedded Tissues: 1/10-1/50. The use of 4% paraformaldehyde fixation rather than formalin fixation is recommended. A sensitive detection system is also recommended, e.g. a catalysed signal amplification system. Does not require protein digestion pre-treatment nor antigen retrieval using heat prior to staining of paraffin embedded sections.
  Suitable Positive Control: Kidney, cultered osteoclasts.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from C-terminal sequence of Rat Calcitonin Receptor
• Specificity: SP1338P reacts with the Calcitonin Receptor.
  It recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b Rat isoforms.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein A.
• Gene Name: Rattus norvegicus calcitonin receptor (Calcr), transcript variant 1a
• Database Link:
  Entrez Gene 799 HumanEntrez Gene 12311 MouseEntrez Gene 116506 Rat
• Background: Calcitonin Receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embyro and may be important in foetal morphogenesis.
• Synonyms: CTR, CT-R, CALCR
• Note: Antiserum Preparation: Antisera to calcitonin receptor were raised by repeated immunisations of Rabbits with highly purified antigen.