Interferon alpha (IFN alpha) Rabbit Polyclonal Antibody

CAT#: SP2126A

Interferon alpha (IFN alpha) rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, FN, IP, WB
• Recommended Dilution: ELISA.
  Western Blot: This antibody cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma; this antibdoy detects a band of approximately 19kDa.
  Functional Assays.
  Immunoprecipitation.
• Reactivities: Chicken
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant chicken Interferon alpha.
• Specificity: This antibody recognizes Interferon alpha (chIFN-alpha). In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities. Product SA027 is the immunogen used to produce this antibody.
• Formulation: PBS
  State: Azide Free
  State: Liquid purified IgG
  Stabilizer: None
  Preservative: None
• Concentration: 0.5 mg/ml
• Purification: Affinity chromatography on protein G
• Background: The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
• Synonyms: B Cell Interferon, IFN-alpha, IFNA, Interferon-alpha, Leukocyte Interferon, Type I Interferon