LOX 1 (OLR1) Sheep Polyclonal Antibody

CAT#: SP2139P

LOX 1 (OLR1) sheep polyclonal antibody, Purified

Product Images

Staining Human Umbilical Vein Endothelial Cells with Sheep anti-LOX-1 Antibody

Specifications

Product Data

• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 10⁶ cells in 100 µl.
  Western Blot: 1/10-1/100. This antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting.
  Immunoprecipitation: Neat.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Human
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Recombinant Human extracellular domain LOX-1 protein from bacteria
• Specificity: This antibody recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1).
  SP2139P will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: oxidized low density lipoprotein receptor 1
• Database Link:
  Entrez Gene 4973 Human
• Background: LOX-1 is a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (1,4) and inflammatory diseases such as atherosclerosis (1) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs).
• Synonyms: LOX-1, Ox-LDL receptor 1, Ox-LDL receptor 1 soluble form, sLOX1, sLOX-1, CLEC8A