Phosphothreonine Rabbit Monoclonal Antibody [Clone ID: RM102]

CAT#: TA160004

Rabbit Monoclonal Antibody against Phosphothreonine

Product Images

Western blot of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using anti-Phosphothreonine antibody at 1/2000 dilution.

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  Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using anti-Phosphothreonine antibody at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

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  Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by anti-Phosphothreonine antibody at 1/500 dilution, was then blotted with anti-Phosphothreonine antibody. (1) whole lysate control; (2)IP by rabbit IgG control; (3) IP by RM111

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  Rabbit monoclonal anti-Phosphothreonine antibody recognizes Phosphorylated threonine in peptides with different sequences. It has minimum cross-reactivity with phosphorylated serine.

Specifications

Product Data

• Clone Name: RM102
• Applications: Assay, IHC, WB
• Recommended Dilution: WB: 1:500 -1:2000, IP: 1:100-1:500, ChIP: 1:100-1:500, ICC: 1:100-1:500, IHC: 1:100-1:500
• Reactivities: All Species
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Note: RM102 reacts threonine-phosphorylated proteins. No cross reactivity with n