Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone ID: RM137]

CAT#: TA160007

Rabbit Monoclonal Antibody against Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)

Product Images

Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using anti-H3K4me3 antibody at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells.

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  A Peptide dotblot shows anti-H3K4me3 antibody only reacts to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).

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  Anti-H3K4me3 antibody specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.

Specifications

Product Data

• Clone Name: RM137
• Applications: Assay, Dot, WB
• Recommended Dilution: WB: 0.2 ug/mL - 1 ug/mL;, ELISA: 0.1 ug/mL - 0.5 ug/mL;, Multiplex: 0.1 ug/mL - 0.5 ug/mL.
• Reactivities: All Species
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Note: RM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross rea