Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [Clone ID: RM175]

CAT#: TA160018

Rabbit Monoclonal Antibody against Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)

Product Images

Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using anti-H3K27me3 antibody at 1 ug/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.

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  Anti-H3K27me3 antibody specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histon

Specifications

Product Data

• Clone Name: RM175
• Applications: Assay, WB
• Recommended Dilution: WB: 1 ug/mL - 2 ug/mL;, ELISA: 0.5ug/mL - 1 ug/mL;, Multiplex: 0.1 ug/mL - 0.5 ug/mL.
• Reactivities: All Species
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Note: RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross r