Phosphotyrosine Rabbit Monoclonal Antibody [Clone ID: RM111]

CAT#: TA160022

Rabbit Monoclonal Antibody against Phosphotyrosine

Product Images

Western blot of serum-starved A431 cells nontreated or treated with EGF, using anti-Phosphotyrosine antibody at 1/5000 dilution. The blot was exposed to films at different time points.

• 

  Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using anti-Phosphotyrosine antibody at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

• 

  Immunoprecipitation of EGF-treated A431 cells by anti-Phosphotyrosine antibody at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) whole lysate control; (2) IP by rabbit IgG control; (3) IP by anti-Phosphotyrosine antibody

• 

  Luminex analysis of anti-Phosphotyrosine antibody reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Anti-Phosphotyrosine antibody only reacted to phosphorylated Tyrosine

Specifications

Product Data

• Clone Name: RM111
• Applications: IHC, LMNX, WB
• Recommended Dilution: WB: 1:1000 -1:5000, IP: 1:200-1:1000, ChIP: 1:200-1:1000, ICC: 1:200-1:500, IHC: 1:200-1:500
• Reactivities: All Species
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Phosphotyrosine-BSA conjugate
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Note: RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with