PKM2 Mouse Monoclonal Antibody [Clone ID: OTI1F12]

CAT#: TA190260

PKM2 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKM2 monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PKM2 mouse monoclonal antibody. (TA190260)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190260)

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Specifications

Product Data

• Clone Name: OTI1F12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: LRRLAPITSDPTEATAVGAV, corresponding to the region encoded by PKM gene M2 isoform.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.