DAXX Rabbit Monoclonal Antibody [Clone ID: E94]

CAT#: TA300370

Rabbit Monoclonal Antibody against DAXX (Clone E94)

Product Images

Western blot - Daxx antibody [E94]; Anti-Daxx antibody [E94] at 1/5000 dilution + Hela cell lysate.Predicted band size : 81 kDa.Observed band size : 100 kDa.Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

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  Immunohistochemistry (Paraffin-embedded sections) - Daxx antibody [E94]; Ab32140, at a dilution of 1/50, staining Daxx in paraffin embedded human stomach adenocarcinoma tissue by Immunohistochemistry.

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  Flow Cytometry - Anti-Daxx antibody; Overlay histogram showing HeLa cells stained with TA300370 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

Specifications

Product Data

• Clone Name: E94
• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:50; FC: 1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues of human DAXX was used as immunogen. Predicted to cross-react with rat, based on sequence homology.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: death-domain associated protein
• Database Link:
  NP_001341
  Entrez Gene 13163 MouseEntrez Gene 140926 RatEntrez Gene 1616 Human
• Background: Daxx is a nuclear protein that has been found to the enhance Fas-mediated apoptosis and activate the JNK pathway (1). Apoptosis signal-regulating kinase 1 (ASK1) controls the dual function of Daxx as a transcriptional repressor in the nucleus and as a proapoptotic signal mediator in the cytoplasm (2). Daxx associates with various proteins, including promyelocytic leukemia protein (PML) and heat shock protein 27, lending significance to their roles in tumor suppression and Daxx-mediated apoptosis (3,4).
• Synonyms: BING2; DAP6; EAP1
• Note: Is unsuitable for IP.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway