Cytokeratin 8 (KRT8) Rabbit Monoclonal Antibody [Clone ID: E431-2]

CAT#: TA300410

Rabbit Monoclonal Antibody against KRT8 (Clone E431-2)

Product Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S73) antibody [E431-2]; IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 diluted 1/500.

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  IHC of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with TA300410.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 diluted 1/250.

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  Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor#174; 488 conjugated donkey anti-rabbit antibody was used as the secondary.

Specifications

Product Data

• Clone Name: E431-2
• Applications: IF, IHC
• Recommended Dilution: IHC-Fr: Use at an assay dependent concentration; WB: 1:10000; ICC/IF: 1:250; IHC-P: Use at an assay dependent dilution
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55 kDa
• Gene Name: keratin 8
• Database Link:
  NP_002264
  Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 3856 Human
  UniProt ID P05787
• Background: Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the ove
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO
• Note: Is unsuitable for Flow Cyt or IP.

Reference Data

• Protein Families: Druggable Genome