BAD Rabbit Monoclonal Antibody [Clone ID: Y208]

CAT#: TA300475

Rabbit Monoclonal Antibody against BAD (Clone Y208)

Product Images

Western blot - Bad antibody [Y208]; Anti-Bad [Y208] antibody at 1/5000 dilution + Hela cell lysate.Predicted band size : 18 kDa.Observed band size : 23 kDa.

• 

  Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208]; Immunohistochemical staining of paraffin-embedded human lymphoma using TA300475 at 1/100 dilution.

• 

  ICC/IF image of TA300475 stained MCF7 cells. The cells were incubated with the antibody overnight at 4. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

• 

  Flow Cytometry-Bad antibody (TA300475); Overlay histogram showing MCF-7 cells stained with TA300475 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells under the same conditions.

Specifications

Product Data

• Clone Name: Y208
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20 - 1:50; IP: 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: BCL2 associated agonist of cell death
• Database Link:
  NP_116784
  Entrez Gene 12015 MouseEntrez Gene 64639 RatEntrez Gene 483763 DogEntrez Gene 572 Human
  UniProt ID Q92934
• Background: Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.
• Synonyms: BBC2; BCL2L8

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway