Calmodulin (CALM2) Rabbit Monoclonal Antibody [Clone ID: EP799Y]

CAT#: TA300636

Rabbit Monoclonal Antibody against CALM2 (Clone EP799Y)

Product Images

Western blot - Calmodulin antibody [EP799Y]; Anti-Calmodulin antibody [EP799Y] at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane).Predicted band size : 17 kDa.Observed band size : 16 kDa.

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  Immunohistochemistry (Paraffin-embedded sections) - Calmodulin antibody [EP799Y]; Ab45689 (1:250) staining human Calmodulin in human urinary bladder carcinoma by immunohistochemistry in paraffin embedded tissue.

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  ICC/IF image of TA300636 stained MCF7 cells. The cells were incubated with the antibody overnight at 4. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

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  Flow Cytometry - Anti-Calmodulin antibody; Overlay histogram showing MCF-7 cells stained with TA300636 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Specifications

Product Data

• Clone Name: EP799Y
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:50; IP: 1:50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in the C-terminus of human Calmodulin was used as immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17 kDa
• Gene Name: calmodulin 2 (phosphorylase kinase, delta)
• Database Link:
  NP_001734
  Entrez Gene 12314 MouseEntrez Gene 50663 RatEntrez Gene 805 Human
• Background: Calmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).
• Synonyms: caM; CAMII; LQT15; PHKD; PHKD2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction