EEF2K Rabbit Monoclonal Antibody [Clone ID: EP724Y]

CAT#: TA300638

Rabbit Monoclonal Antibody against EEF2K (Clone EP724Y)

Product Images

Western blot - EEF2K antibody [EP724Y]; All lanes : Anti-EEF2K antibody [EP724Y] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 95 kDa.

Specifications

Product Data

• Clone Name: EP724Y
• Applications: WB
• Recommended Dilution: WB: 1:50000; IP: 1:20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in the Calmodulin binding region of human eEF2 was used as immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82 kDa
• Gene Name: eukaryotic elongation factor 2 kinase
• Database Link:
  NP_037434
  Entrez Gene 13631 MouseEntrez Gene 25435 RatEntrez Gene 29904 Human
  UniProt ID O00418
• Background: Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6).
• Synonyms: eEF-2K; HSU93850
• Note: Is unsuitable for Flow Cyt,ICC or IHC-P.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase