MEK4 (MAP2K4) Rabbit Monoclonal Antibody [Clone ID: EP1075Y]

CAT#: TA300743

Rabbit Monoclonal Antibody against MAP2K4 (Clone EP1075Y)

Product Images

Western blot - MKK4 (phospho S80) antibody [EP1075Y]; All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] at 1/5000 dilution.Lane 1 : 3T3 cell lysate untreated.Lane 2 : 3T3 cell lysate treated with PDGF.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 44 kDa.Observed band size : 44 kDa.

• 

  Immunohistochemistry (Paraffin-embedded sections) - MKK4 (phospho S80) antibody [EP1075Y]; TA300743 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.

Specifications

Product Data

• Clone Name: EP1075Y
• Applications: IHC, WB
• Recommended Dilution: WB: 1:5000; IHC: 1:50 100; IP: 1:50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues near the N-term of human MKK4 was used as immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44 kDa
• Gene Name: mitogen-activated protein kinase kinase 4
• Database Link:
  NP_003001
  Entrez Gene 26398 MouseEntrez Gene 287398 RatEntrez Gene 6416 Human
  UniProt ID P45985
• Background: MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).
• Synonyms: JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1
• Note: Does not react with Rat

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway