LDHA Rabbit Monoclonal Antibody [Clone ID: EP1563Y]

CAT#: TA300879

Rabbit Monoclonal Antibody against LDHA (Clone EP1563Y)

Product Images

Western blot - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Anti-Lactate Dehydrogenase antibody [EP1563Y] at 1/1000 dilution + A549 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 37 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma labelling Lactate Dehydrogenase with TA300879 at 1/100 dilution.

Specifications

Product Data

• Clone Name: EP1563Y
• Applications: IHC, WB
• Recommended Dilution: ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; IP: 1:70; IHC-P: 1:100 - 1:250; FC: 1:30
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in human LDH-A was used as immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37 kDa
• Gene Name: lactate dehydrogenase A
• Database Link:
  NP_005557
  Entrez Gene 16828 MouseEntrez Gene 24533 RatEntrez Gene 3939 Human
  UniProt ID P00338
• Background: Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
• Synonyms: GSD11; HEL-S-133P; LDHM; PIG19

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism