S100 beta (S100B) Rabbit Monoclonal Antibody [Clone ID: EP1576Y]

CAT#: TA300915

Rabbit Monoclonal Antibody against S100B (Clone EP1576Y)

Product Images

Western blot - S100 beta antibody [EP1576Y]; Anti-S100 beta antibody [EP1576Y] at 1/1000 dilution + A375 cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.

• 

  TA300915 stainingS100 betainHuman spiral gangliontissue sections by IHC. Tissue was fixed with paraformaldehyde and blocked with 1%BSA for 30 minutes at RT; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

• 

  Immunohistochemistry (Paraffin-embedded sections) - S100 beta antibody [EP1576Y]; Ab52642 (1/100) staining human S100 beta in human melanoma tissue by immunohistochemistry using paraffin embedded tissue.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - S100 beta antibody [EP1576Y]; TA300915 at 1/500 staining human melanoma tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed, before being blocked and incubated with the antibody for 1 hour. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-S100 beta antibody [EP1576Y] (TA300915); TA300915 showing positive staining in Breast carcinoma tissue.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-S100 beta antibody [EP1576Y] (TA300915); TA300915 showing positive staining in Glioma tissue.

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y]; TA300915 showing positive staining in Clear cell carcinoma of kidney tissue.

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y]; Immunohistochemical analysis of embryonic mouse brain tissue, staining S100 beta with TA300915.

• Other-Anti-S100 beta antibody [EP1576Y] (TA300915); Equilibrium disassociation constant (KD).

Show all 9 images

Specifications

Product Data

• Clone Name: EP1576Y
• Applications: Assay, IHC, WB
• Recommended Dilution: ICC/IF: Use at an assay dependent dilution; WB: 1:1000; IP: 1:40; IHC-P: Use at an assay dependent dilution
• Reactivities: Mouse, Rat, Goat, Human, ZebraFish, Macaque Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human S100-β was used as an immunogen.
• Formulation: PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purification: Tissue culture supernatant
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11 kDa
• Gene Name: S100 calcium binding protein B
• Database Link:
  NP_006263
  Entrez Gene 20203 MouseEntrez Gene 25742 RatEntrez Gene 6285 Human
• Background: S100-β is a homodimeric member of the EF-hand calcium-binding protein superfamily. The protein has been implicated in cellular processes such as cell differentiation and growth, plays a role in cytoskeletal structure and function, and may have a role in neuropathological diseases, such as Alzheimers. The effects of S100-β are mediated via its interaction with target proteins (1). S100-β protein is a low molecular weight, EF-hand, Ca2(+)-binding protein widely distributed and conserved in the central nervous system of vertebrates (2). Measurements of Ca2+ binding to human brain S100-β (beta beta) protein revealed six Ca2+-binding sites and zinc-binding studies on human S100b protein showed that the protein bound two zinc ions per beta monomer. These results indicate that the Ca2+- and Zn2+-binding sites on S100-β protein are different and suggest that Zn2+ may regulate Ca2+ binding by increasing the affinity of the protein for calcium (3).
• Synonyms: NEF; S100; S100-B; S100beta
• Note: Is unsuitable for Flow Cyt.