Cyclin D1 (CCND1) Rabbit Monoclonal Antibody [Clone ID: EPR2241]

CAT#: TA301123

Rabbit Monoclonal Antibody against CCND1 (Clone EPR2241IHC)

Product Images

Western blot - Anti-Cyclin D1 antibody [EPR2241];.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 34 kDa.Exposure time : 3 minutes

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  Western blot - Anti-Cyclin D1 antibody [EPR2241]; Anti-Cyclin D1 antibody [EPR2241] at 1/10000 dilution + MCF7 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EPR2241]; Immunohistochemical analysis of paraffin-embedded Human mantle cell lymphoma tissue labelling Cyclin D1 with TA301123 at 1/100 dilution.

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  Other-Anti-Cyclin D1 antibody [EPR2241] (TA301123); Equilibrium disassociation constant (KD).

Specifications

Product Data

• Clone Name: EPR2241
• Applications: Assay, IHC, WB
• Recommended Dilution: WB: 1:10000; IP: 1:40; ICC/IF: 1:100; IHC-P: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues near the C-terminus of human Cyclin D1 was used as an immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: cyclin D1
• Database Link:
  NP_444284
  Entrez Gene 12443 MouseEntrez Gene 58919 RatEntrez Gene 595 Human
• Background: Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition (1). Cyclin D1 forms a serine/threonine kinase holoenzyme complex with and functions as a regulatory subunit of CDK4 or CDK6. Cyclin D1 also phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1/S phase (2-3). Amplification or overexpression of cyclin D1 plays a pivotal role in the development of various human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer (4). Over-expression of cyclin D1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis (5). Cyclin D1 also associates with, and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins (6-7)
• Synonyms: BCL1; D11S287E; PRAD1; U21B31
• Note: Is unsuitable for Flow Cyt.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Focal adhesion, Glioma, Jak-STAT signaling pathway, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Viral myocarditis, Wnt signaling pathway