alpha Actinin (ACTN1) Rabbit Monoclonal Antibody [Clone ID: EP2527Y]

CAT#: TA301137

Rabbit Monoclonal Antibody against ACTN1 (Clone EP2527Y)

Product Images

Western blot - alpha Actinin antibody [EP2527Y]; All lanes : Anti-alpha Actinin antibody [EP2527Y] at 1/10000 dilution.Lane 1 : NIH 3T3 lysate.Lane 2 : HeLa lysate.Lane 3 : C6 lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 103 kDa.Observed band size : 103 kDa.

Specifications

Product Data

• Clone Name: EP2527Y
• Applications: WB
• Recommended Dilution: WB: 1:2,000; IHC: 1: 100; FC: 1:80; IP: 1:40
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 1 was used as an immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 103 kDa
• Gene Name: actinin alpha 1
• Database Link:
  NP_001093
  Entrez Gene 81634 RatEntrez Gene 109711 MouseEntrez Gene 87 Human
  UniProt ID P12814
• Background: Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).
• Synonyms: BDPLT15

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction