Aromatase (CYP19A1) Rabbit Polyclonal Antibody

CAT#: TA301409

Rabbit Polyclonal Antibody against Aromatase

Product Images

Immunocytochemistry/Immunofluorescence: Aromatase Antibody TA301409 - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aromatase Antibody TA301409 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Western Blot: Aromatase Antibody TA301409 - Detection of aromatase in human fetal temporal lobe lysate using NB 100-1596. ECL exposure, 1-2 minutes.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1 ug/ml, Immunohistochemistry: 1:200, Simple Western, Immunohistochemistry-Paraffin: 1:200
• Reactivities: Human, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal portion of the human aromatase protein sequence (between residues 400-502).
• Formulation: Tris-citrate / phosphate [pH 7-8] and 0.1% sodium azide
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cytochrome P450 family 19 subfamily A member 1
• Database Link:
  NP_000094
  Entrez Gene 678697 MonkeyEntrez Gene 1588 Human
  UniProt ID P11511
• Background: Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
• Synonyms: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways