PI 3 Kinase Class 3 (PIK3C3) Rabbit Polyclonal Antibody

CAT#: TA301456

Rabbit Polyclonal Antibody against VPS34

Product Images

Immunocytochemistry/Immunofluorescence: VPS34 Antibody TA301456 - VPS34 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

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  Simple Western: VPS34 Antibody TA301456 - Simple Western lane view shows a specific band for VPS34 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: VPS34 Antibody TA301456 - HepG2 whole cell lysates.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Simple Western: 1:20, Knockdown Validated
• Reactivities: Human, Rat, Mouse, Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to an internal portion of the human protein (within residues 700-850). [Swiss-Prot# Q8NEB9]
• Formulation: PBS, 0.1% BSA, 50% glycerol and 0.05% Sodium Azide
• Purification: peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• Database Link:
  NP_002638
  Entrez Gene 65052 RatEntrez Gene 225326 MouseEntrez Gene 5289 Human
  UniProt ID Q8NEB9
• Background: VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.
• Synonyms: hVps34; VPS34; Vps34

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy