Stromal interaction molecule 1 (STIM1) Rabbit Polyclonal Antibody

CAT#: TA301466

Rabbit Polyclonal Antibody against STIM-1

Product Images

Immunocytochemistry/Immunofluorescence: STIM1 Antibody TA301466 - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody.

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  Western Blot: STIM1 Antibody TA301466 - Stromal interaction molecule 1 Antibody TA301466 - Detection of STIM1 in HeLa whole cell extracts.

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  Knockdown Validated: STIM1 Antibody TA301466 - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stim-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Stim-1 Polyclonal Antibody (Catalog # TA301466) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stim-1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Western Blot: 1:1000, Knockdown Validated: 1:1000
• Reactivities: Human, Mouse, Rat, Bovine, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal region (within residues 600-685) of the human protein. [Uniprot# Q13586]
• Formulation: PBS + 30% glycerol and 0.1% sodium azide
• Concentration: lot specific
• Purification: peptide affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: stromal interaction molecule 1
• Database Link:
  NP_003147
  Entrez Gene 20866 MouseEntrez Gene 361618 RatEntrez Gene 608643 DogEntrez Gene 6786 Human
  UniProt ID Q13586
• Background: Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1

Reference Data

• Protein Families: Transmembrane