PODXL Mouse Monoclonal Antibody [Clone ID: TRA-1-60(R)]

CAT#: TA301484

Mouse Monoclonal Antibody against TRA-1-60 (TRA-1-60(R)) -Neuraminidase resistant -Embryonic Stem Cell Marker

Product Images

Immunocytochemistry/Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) TA301484 - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# TA301484) detected with Alexa Fluor 488 anti-mouse IgM secondary antibody.

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  Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) TA301484 - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # TA301484, filled histogram) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).

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  Immunocytochemistry/Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) TA301484 - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Mouse Anti-Human TRA-1-60(R) Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # TA301484) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.

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  Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) TA301484 - Analysis of TA301484 expression in NTERA-2 cl.D1 whole cell lysate.

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  Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) TA301484 - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells.

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Specifications

Product Data

• Clone Name: TRA-1-60(R)
• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 1:100-1:2000, ELISA, Immunohistochemistry-Paraffin, Flow Cytometry: 1:50-1:200, Immunohistochemistry: 1:50-1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human embryonal carcinoma cell line 2102Ep.
• Formulation: Tris-glycine, 150 mM NaCl, 0.05% sodium azide
• Concentration: lot specific
• Purification: IgM purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: podocalyxin like
• Database Link:
  NP_005388
  Entrez Gene 5420 Human
  UniProt ID O00592
• Background: TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.
• Synonyms: Gp200; PC; PCLP; PCLP-1

Reference Data

• Protein Families: Transmembrane