PARK7 Rabbit Polyclonal Antibody

CAT#: TA301508

Rabbit Polyclonal Antibody against DJ-1

Product Images

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody TA301508 - Park7(DJ-1) detected in paraffin embedded human cortex.

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  Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Park7/DJ-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

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  Simple Western: Park7/DJ-1 Antibody TA301508 - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: Park7/DJ-1 Antibody TA301508 - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).

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  Western Blot: Park7/DJ-1 Antibody TA301508 - Human HEK293 cells and mouse brain lysates.

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Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1:2000, Simple Western: 1:4000, Immunocytochemistry/ Immunofluorescence: 1:500, Immunoprecipitation: 1:200, In vitro assay, Immunohistochemistry-Paraffin, Flow Cytometry
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to residues between 150-189 of human DJ-1 (the C-terminus).
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Parkinsonism associated deglycase
• Database Link:
  NP_009193
  Entrez Gene 57320 MouseEntrez Gene 11315 Human
  UniProt ID Q99497
• Background: DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinsonism.
• Synonyms: DJ-1; DJ1; HEL-S-67p

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Parkinson's disease