Bcl2 Binding component 3 (BBC3) Rabbit Polyclonal Antibody

CAT#: TA301519

Rabbit Polyclonal Antibody against PUMA

Product Images

Immunocytochemistry/Immunofluorescence: PUMA Antibody TA301519 - The PUMA antibody was tested at a 1:20 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

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  Western Blot: PUMA Antibody TA301519 - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:20-1:100, Western Blot: 2-4 ug/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193).
• Formulation: Tris-glycine, 150 mM NaCl, 0.05% sodium azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2 binding component 3
• Database Link:
  NP_055232
  Entrez Gene 27113 Human
  UniProt ID Q96PG8
• Background: A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
• Synonyms: JFY-1; JFY1; PUMA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease, p53 signaling pathway