EGLN1 Rabbit Polyclonal Antibody

CAT#: TA301539

Rabbit Polyclonal Antibody against HIF Prolyl Hydroxylase 2

Product Images

Western blot analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.

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  Western Blot: HIF Prolyl Hydroxylase 2 Antibody - Detection of Human PHD2 by Western Blot. Samples: Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells, stained with rabbit anti-PHD2 used at 1 ug/ml.

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  Flow Cytometry: HIF Prolyl Hydroxylase 2 Antibody - Flow cytometric detection of PHD2, 10^6 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 in a 150 ul reaction.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1: 1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal fragment of the human protein sequence of HIF prolyl hydroxylase 2 (residues 350-426).
• Formulation: Tris-citrate/phosphate, pH 7-8, 0.09% sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: egl-9 family hypoxia inducible factor 1
• Database Link:
  NP_071334
  Entrez Gene 54583 Human
  UniProt ID Q9GZT9
• Background: HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
• Synonyms: C1orf12; ECYT3; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; SM20; ZMYND6

Reference Data

• Protein Pathways: Pathways in cancer, Renal cell carcinoma