Wilms Tumor Protein (WT1) Mouse Monoclonal Antibody [Clone ID: 6F-H2]

CAT#: TA301555

Mouse Monoclonal Antibody against Wilms Tumor 1 (6F-H2)

Product Images

Simple Western: WT1 Antibody (6F-H2) TA301555 - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: WT1 Antibody (6F-H2) TA301555 - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry: WT1 Antibody (6F-H2) TA301555 - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.

Specifications

Product Data

• Clone Name: 6F-H2
• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen: 1: 400, Immunohistochemistry-Paraffin: 1:400, Simple Western: 1:50, Western Blot: 2 ug/ml, Immunoprecipitation: 2-10 ug/ml lysate, Immunohistochemistry: 1:400
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Human recombinant WT1 protein (residues 1-181). [Swiss-Prot# P19544]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% sodium azide
• Purification: IgG purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Wilms tumor 1
• Database Link:
  NP_077744
  Entrez Gene 7490 Human
  UniProt ID P19544
• Background: Wilms?tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms?tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms?tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
• Synonyms: AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33

Reference Data

• Protein Families: Druggable Genome, Transcription Factors