DLX4 Rabbit Polyclonal Antibody

CAT#: TA301565

Rabbit Polyclonal Antibody against BP1

Product Images

Immunohistochemistry-Frozen: BP1 Antibody TA301565 - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.

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  Immunoprecipitation: BP1 Antibody TA301565 - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (TA301565) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.

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  Western Blot: BP1 Antibody TA301565 - Detection of MDA-MB-231 cell lysates.

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  Effect of BP1 on TNFα-induced cell death. (a) Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:50-1:100, Immunoprecipitation: 1:10-1:500, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:50-1:100, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made specifically to the human BP1 protein sequence (between residues 1-60).There is no homology with the peptide sequences used for immunogen of this antibody and the DLX4 or DLX7 proteins.
• Formulation: Tris-citrate/phosphate, pH 7-8, 0.1% sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26 kDa
• Gene Name: distal-less homeobox 4
• Database Link:
  NP_612138
  Entrez Gene 1748 Human
  UniProt ID Q92988
• Background: DLX (Distal-less) genes are essential for early development. BP1 (beta protein 1) is a homeodomain-containing isoform of DLX4. Strong BP1 expression is seen in placenta and kidney tissue, with no expression found in any other tissue (1). This protein has been shown to be activated in 80% of invasive ductal breast tumors and is associated with breast cancer progression ( 2, 3). It is also expressed in the bone marrows of 63% of acute myeloid leukemia (AML) patients (4). In addition, BP1 plays a role in globin gene regulation by repressing the beta- globin gene (1).
• Synonyms: BP1; DLX7; DLX8; DLX9; OFC15

Reference Data

• Protein Families: Transcription Factors