Telomerase reverse transcriptase (TERT) Mouse Monoclonal Antibody [Clone ID: 2C4]

CAT#: TA301588

Mouse Monoclonal Antibody against Telomerase reverse transcriptase (2C4) - Embryonic Stem Cell Marker

Product Images

Immunohistochemistry-Paraffin: TERT Antibody (2C4) TA301588 - Human Pancreatic carcinoma showing strong staining of tumor cells.

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  Flow Cytometry: TERT Antibody (2C4) TA301588 - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) TA301588 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.

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  Immunohistochemistry-Paraffin: TERT Antibody (2C4) TA301588 - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.

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  Flow Cytometry: TERT Antibody (2C4) TA301588 - An intracellular stain was performed on A431 cells with TERT Antibody [2C4] TA301588 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgM (H+L) Cross-Adsorbed Secondary Antibody, AlexaFluor 488.

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  Western Blot: TERT Antibody (2C4) TA301588 - 1:500 dilution on MJ90 cells.

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Specifications

Product Data

• Clone Name: 2C4
• Applications: FC, IHC, WB
• Recommended Dilution: Flow Cytometry: 1:50 - 1:200, Western Blot: 1:500, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Chromatin Immunoprecipitation, Immunohistochemistry: 1:50, Immunohistochemistry-Paraffin: 1:50, Chromatin Immunoprecipitation (ChIP)
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Full-length recombinant hTERT from insect cells.
• Formulation: 0.05% sodium azide
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: telomerase reverse transcriptase
• Database Link:
  NP_937983
  Entrez Gene 7015 Human
  UniProt ID O14746
• Background: Telomeres are nucleoprotein structures at the ends of eukaryotic chromosomes. They regulate several crucial cellular functions and participate in the control of complex phenotypes, such as aging and cancer. Cell immortalization is strongly associated with the stabilization of telomere length. Therefore, it is suggested that cancer cells achieve immortalization in part through illegitimate activation of telomerase expression. hTERT is the rate-limiting catalytic subunit of telomerase. Telomerase is an enzyme uniquely associated with immortal cell lines and is useful for identifying undifferentiated PSCs.
• Synonyms: CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TP2; TRT

Reference Data

• Protein Families: Druggable Genome