ROBO4 Rabbit Polyclonal Antibody

CAT#: TA301644

Rabbit Polyclonal Antibody against ROBO4 - Tumor Endothelial Marker

Product Images

Immunohistochemistry: Robo4 Antibody TA301644 - Detection of ROBO4 in human placental epithelial cells and trophoblasts.

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  Immunocytochemistry/Immunofluorescence: Robo4 Antibody TA301644 - Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Western Blot: Robo4 Antibody TA301644 - Detection of ROBO4 in HUVEC lysate.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Immunohistochemistry: 5 ug/ml, Western Blot: 1:500, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal region of the human protein (within residues 900-1007). [Swiss-Prot# Q8WZ75]
• Formulation: Tris-citrate/phosphate [pH 7-8] and 0.1% sodium azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: roundabout guidance receptor 4
• Database Link:
  NP_061928
  Entrez Gene 54538 Human
  UniProt ID Q8WZ75
• Background: Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development. ROBO4 is a novel roundabout protein which is restricted in expression to endothelial cells in vitro and sites of angiogenesis in vivo. Because it is not expressed on normal endothelial cells, in vivo, it is a promising tumor endothelial marker.
• Synonyms: ECSM4; MRB

Reference Data

• Protein Families: Druggable Genome