EIF4EL3 (EIF4E2) Rabbit Polyclonal Antibody

CAT#: TA302039

Rabbit Polyclonal Antibody against EIF4E2 (N-term)

Product Images

Western blot analysis of EIF4E2 Antibody (N-term) polyclonal antibody (Cat.#TA302039) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4E2 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody (N-term) (Cat.#TA302039), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This EIF4E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human EIF4E2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28362 Da
• Gene Name: eukaryotic translation initiation factor 4E family member 2
• Database Link:
  NP_004837
  Entrez Gene 9470 Human
  UniProt ID O60573
• Background: EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Synonyms: 4E-LP; 4EHP; EIF4EL3; IF4e

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway