PDK4 Rabbit Polyclonal Antibody

CAT#: TA302046

Rabbit Polyclonal Antibody against PDK4 (C-term)

Product Images

The PDK4 Antibody (C-term) (Cat# TA302046) is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.

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  PDK4 Antibody (C396) (Cat. #TA302046) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PDK4 antibody detected the PDK4 protein (arrow).

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  Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Formalin-fixed and paraffin-embedded human Adrenal tissue reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  IF image of HeLa cells stained with PDK4 (C-term) antibody. HeLa cells were incubated with TA302046 PDK4 (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the PDK4 mainly to the cytoplasm.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, IHC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46469 Da
• Gene Name: pyruvate dehydrogenase kinase 4
• Database Link:
  NP_002603
  Entrez Gene 27273 MouseEntrez Gene 5166 Human
  UniProt ID Q16654
• Background: PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
• Synonyms: FLJ40832

Reference Data

• Protein Families: Druggable Genome, Protein Kinase