SMAD4 Rabbit Polyclonal Antibody

CAT#: TA302055

Rabbit Polyclonal Antibody against SMAD4 (T277)

Product Images

Western blot analysis of SMAD4 Antibody (T277) (arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat.#TA302055). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies).

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  Confocal immunofluorescent analysis of SMAD4 Antibody (T277) (Cat#TA302055) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60439 Da
• Gene Name: SMAD family member 4
• Database Link:
  NP_005350
  Entrez Gene 17128 MouseEntrez Gene 50554 RatEntrez Gene 4089 Human
  UniProt ID Q13485
• Background: SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
• Synonyms: DPC4; JIP; MADH4; MYHRS

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway