CTDSP1 Rabbit Polyclonal Antibody

CAT#: TA302078

Rabbit Polyclonal Antibody against CTDSP1 (C-term)

Product Images

The anti-CTDSP1-V250 (Cat. #TA302078) is used in Western blot to detect CTDSP1-V250 in CEM tissue lysate.

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  Western blot analysis of CTDSP1 (arrow) using CTDSP1-V250 Antibody (C-term) (Cat.#TA302078). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTDSP1 gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29203 Da
• Gene Name: CTD small phosphatase 1
• Database Link:
  NP_872580
  Entrez Gene 227292 MouseEntrez Gene 58190 Human
  UniProt ID Q9GZU7
• Background: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.
• Synonyms: NIF3; NLI-IF; NLIIF; SCP1

Reference Data

• Protein Families: Phosphatase