GFAP Rabbit Polyclonal Antibody

CAT#: TA302094

Rabbit Polyclonal Antibody against GFAP (N-term)

Product Images

Western blot analysis of GFAP (arrow) using GFAP Antibody (N-term) (Cat.#TA302094). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC: 1:50~100, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This GFAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human GFAP.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49880 Da
• Gene Name: glial fibrillary acidic protein
• Database Link:
  NP_002046
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.
• Synonyms: ALXDRD

Reference Data

• Protein Families: ES Cell Differentiation/IPS