PDK2 Rabbit Polyclonal Antibody

CAT#: TA302110

Rabbit Polyclonal Antibody against PDK2 (C-term)

Product Images

Western blot analysis of PDK2 Antibody (C-term) (Cat. #TA302110) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab.

• 

  Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term) (Cat. #TA302110). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).

• 

  PDK2 Antibody (C392) (Cat. #TA302110) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).

• 

  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 amino acids from the C-terminal region of human PDK2.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46153 Da
• Gene Name: pyruvate dehydrogenase kinase 2
• Database Link:
  NP_002602
  Entrez Gene 18604 MouseEntrez Gene 81530 RatEntrez Gene 704169 MonkeyEntrez Gene 5164 Human
  UniProt ID Q15119
• Background: PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
• Synonyms: PDHK2; PDKII

Reference Data

• Protein Families: Druggable Genome, Protein Kinase