alpha 1 Fetoprotein (AFP) Rabbit Polyclonal Antibody

CAT#: TA302117

Rabbit Polyclonal Antibody against AFP (Center)

Product Images

Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) (Cat.#TA302117). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) (Cat.#TA302117), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the Central region of human AFP.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68678 Da
• Gene Name: alpha fetoprotein
• Database Link:
  NP_001125
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Synonyms: AFPD; FETA; HPAFP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein