NANOG Rabbit Polyclonal Antibody

CAT#: TA302154

Rabbit Polyclonal Antibody against NANOG (Center)

Product Images

NANOG Antibody (Center) (Cat. #TA302154) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).

• 

  Formalin-fixed and paraffin-embedded human Testis tissue reacted with NANOG antibody (Center) (Cat.#TA302154), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

• 

  Immunofluorescence analysis of NANOG Antibody (Center) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.

• 

  Flow cytometric analysis of hela cells using NANOG Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human NANOG.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34489 Da
• Gene Name: Nanog homeobox
• Database Link:
  NP_079141
  Entrez Gene 715746 MonkeyEntrez Gene 79923 Human
  UniProt ID Q9H9S0
• Background: NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
• Synonyms: homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox

Reference Data

• Protein Families: Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency